Screening of Antiparasitic Compounds

All compounds were purchased from Sigma-Aldrich and included: gambogic acid, sodium salinomycin, ethinyl estradiol, fluoxetidine hydrochloride, bepridil, ciclopirox, miconazole nitrate, chlorpromazine hydrochloride, amphotericin b, niclosamide, rescinnamine, flucytosine, vinblastine, carbidopa, praziquantel and auranofin.
Stock solutions of all compounds were made up at 1 mM in appropriate solvents (Dataset S1) and stored at −80°C. All compounds were added to black-sided, flat-bottom (optically clear), 96-well microtiter plates containing schistosomula (1000 parasites/well in triplicate) at 10 µM concentrations. Schistosomula were cultured (as already indicated; 37°C, 5% CO₂) in the presence of each compound for 24 hr before viability levels were assessed.