Antimicrobial Evaluation of Microbial Strains

The studied microorganisms included reference strains of Providencia smartii (ATCC29916), Pseudomonas aeruginosa (PA01), Klebsiella pneumoniae (ATCC11296), Staphylococcus aureus (ATCC25922), Salmonella typhi (ATCC6539), Escherichia coli (ATCC8739 and AG100), Candida albicans (ATCC 9002) obtained from the American Type Culture Collection. They were maintained on agar slant at 4˚C and sub-cultured on a fresh appropriate agar plates 24 h prior to any antimicrobial test. Nutrient Agar and Sabouraud Glucose Agar were used for the activation of bacteria and fungi respectively. The Mueller Hinton Broth (MHB) was used for the MIC and MMC determinations. The Mueller Hinton Agar (MHA) was also used for the determination of the MMC on these species.

Free full text: Click here

Kuete V., Kamga J., Sandjo L.P., Ngameni B., Poumale H.M., Ambassa P, & Ngadjui B.T. (2011). Antimicrobial activities of the methanol extract, fractions and compounds from Ficus polita Vahl. (Moraceae). BMC Complementary and Alternative Medicine, 11, 6.

Publication 2011

Agar Antimicrobial Bacteria Candida albicans Escherichia coli Fungi Glucose Klebsiella pneumoniae Nutrient Providencia Pseudomonas aeruginosa Salmonella typhi Staphylococcus aureus Strains

Corresponding Organization : Université de Dschang

Other organizations : Université de Yaoundé I

Top 5 similar protocols

Protocol cited in 10 other protocols

Variable analysis

independent variables

Microorganisms (reference strains of Providencia smartii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Salmonella typhi, Escherichia coli, and Candida albicans)

dependent variables

Antimicrobial activity
Minimum Inhibitory Concentration (MIC)
Minimum Microbicidal Concentration (MMC)

control variables

Maintenance of microorganisms on agar slants at 4°C
Subculturing of microorganisms on fresh agar plates 24 hours prior to antimicrobial testing
Use of Nutrient Agar and Sabouraud Glucose Agar for activation of bacteria and fungi, respectively
Use of Mueller Hinton Broth (MHB) for MIC and MMC determinations
Use of Mueller Hinton Agar (MHA) for MMC determination

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!