Flow cytometric analysis was done using a LSRII and cell sorting used a Cytopeia Influx (BD Biosciences, Sydney, Australia) equipped as previously described [32 (link)]. For generic analysis, cells were analysed at 10–20,000 cells per second except for cell cycle analysis which were analysed at 2000 cells per second. Cell sorting was performed at ~20,000 cells per second and re-analysed to confirm purity (>95%).
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