Genomic DNA was isolated from freshly collected chicken feces according to the previously established protocol [10 (link)]. Fecal samples were transported directly in a sealed box to the laboratory for DNA extraction and were maintained at less than 4°C during transport. The Power Fecal DNA isolation kit (Mo Bio Laboratories, Inc., Hercules, CA, USA) was utilized for DNA extraction, according to the manufacturer’s instructions. The quality of the purified DNA was checked through agarose gel electrophoresis and a UV/Vis Spectrophotometer (Mecasys Co., Ltd., Daejeon, Korea).
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Fecal DNA Isolation for Microbiome Analysis
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Other organizations : Dankook University, Florida Agricultural and Mechanical University
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Variable analysis
independent variables
- Isolation of genomic DNA from freshly collected chicken feces
- Quality of the purified DNA
- Fecal samples were transported directly in a sealed box to the laboratory and maintained at less than 4°C during transport
- The Power Fecal DNA isolation kit (Mo Bio Laboratories, Inc., Hercules, CA, USA) was utilized for DNA extraction, according to the manufacturer's instructions
- Positive control: Not specified
- Negative control: Not specified
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