Genomic DNA was extracted from fungal mycelia grown on PDA, using a modified CTAB protocol as described in Guo et al. (2000) (link). Seven loci, including the 5.8S nuclear ribosomal RNA gene with the two flanking internal transcribed spacer (ITS) re gions, intergenic spacer region of the rDNA (IGS), partial trans lation elongation factor (tef1), partial calmodulin (cam), partial RNA polymerase largest subunit (rpb1), partial RNA polymerase second largest subunit (rpb2) gene regions, and partial β-tubulin (tub2), were amplified and sequenced, respectively. The primer pairs and PCR amplification procedures following protocols described by O’Donnell et al. (1998a , b (link), 2008 (link), 2009a (link), b (link), 2010 (link)), Crous et al. (2009 (link), 2021 (link)), and Lombard et al. (2015) (link), are listed in Table 2. PCR amplifications were performed in a reaction mixture consisting of 12.5 μL 2 × Taq PCR Master Mix (Vazyme Biotech Co., Ltd, Nanjing, China), 1 μL each of 10 μM primers, 1 μL of the undiluted genomic DNA, adjusted to a final volume of 25 μL with distilled deionized water. The PCR products were visualised on 1 % agarose electrophoresis gel. Sequencing was done bi-directionally, conducted by the Tianyi Huiyuan Company (Beijing, China). Consensus sequences were obtained using SeqMan of the Lasergene software package v. 14.1 (DNAstar, Madison, Wisconsin, USA).