Integrin β1 shRNA plasmid (#sc-29375-SH), Integrin α3 shRNA plasmid (#sc-35684-SH), Integrin α6 shRNA plasmid (#sc-43129-SH), and control shRNA plasmid-A (#sc-108060) were purchased from Santa Cruz Biotechnology for knockdown of the target integrins. The knockdown efficiency was evaluated by western blotting.
The mGFP-paxillin expression vector was a gift from A. Kusumi (Okinawa Institute of Science and Technology, Japan). pGFP-FAK and pEGFP-vinculin were gifts from Kenneth Yamada (RRID: Addgene_50513 and Addgene¬_50515)39 , EGFP-talin, mVenus-Integrin-Beta1 and mRuby-Lifeact-7 was a gift from Michael Davidson (RRID: Addgene_54560). pEGFPC1/Gg Vinculin 1-258 (akaVD1) was a gift from S. Craig (RRID: Addgene_46270)40 (link). The Rac1 FRET Sensor, pCAGGS-RaichuEV-Rac1was generously provided by K. Aoki (National Institute for Basic Biology, Japan)41 (link) and the RhoA FRET sensor, DORA-RhoA was generously provided by Yi I. Wu (University of Connecticut Health Center, USA)42 . The expression vectors for Rac1/Tiam1 activation system (Lyn11-linker-FRB, YFP-FKBP, YFP-FKBP-linker-Tiam1, and pTriEx-PA-Rac1) was generously provided by T. Inoue (Johns Hopkins University, USA)43 (link).
The mGFP-paxillin expression vector was a gift from A. Kusumi (Okinawa Institute of Science and Technology, Japan). pGFP-FAK and pEGFP-vinculin were gifts from Kenneth Yamada (RRID: Addgene_50513 and Addgene¬_50515)39 , EGFP-talin, mVenus-Integrin-Beta1 and mRuby-Lifeact-7 was a gift from Michael Davidson (RRID: Addgene_54560). pEGFPC1/Gg Vinculin 1-258 (akaVD1) was a gift from S. Craig (RRID: Addgene_46270)40 (link). The Rac1 FRET Sensor, pCAGGS-RaichuEV-Rac1was generously provided by K. Aoki (National Institute for Basic Biology, Japan)41 (link) and the RhoA FRET sensor, DORA-RhoA was generously provided by Yi I. Wu (University of Connecticut Health Center, USA)42 . The expression vectors for Rac1/Tiam1 activation system (Lyn11-linker-FRB, YFP-FKBP, YFP-FKBP-linker-Tiam1, and pTriEx-PA-Rac1) was generously provided by T. Inoue (Johns Hopkins University, USA)43 (link).
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