K562 cells stably expressing proinsulin [12 (link)] and HEK293T cells for virus production were cultured in IMDM (Lonza). All media were supplemented with 10% FCS, 100U/mL penicillin, 100U/mL streptomycin and 2mM GlutaMAX. Cell lines were all maintained in humidified incubators at 37⁰C and 5% CO2.
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