Western Blot Analysis for Phospho-Akt Detection

Western blot analysis was carried out essentially as previously described in (Villafañez et al., 2019 (link)). The detection and quantification were performed with Odyssey CLX System (LI-COR Biosciences) using the proprietary Image Studio Software. The following primary antibodies were used: α-phospho-Akt (Ser473; Cell Signaling Technology, Cat# 9271); anti-β-Actin was from (Sigma-Aldrich, Cat# A2228). As secondary antibodies we used: goat α-mouse IRDye 680RD (LI-COR Biosciences, Cat# P/N 925-68070) and goat α-rabbit IRDye 800CW (LI-COR Biosciences, Cat# P/N 925-32211).

Free full text: Click here

García I.A., Pansa M.F., Pacciaroni A.D., García M.E., Gonzalez M.L., Oberti J.C., Bocco J.L., Carpinella M.C., Barboza G.E., Nicotra V.E, & Soria G. (2020). Synthetic Lethal Activity of Benzophenanthridine Alkaloids From Zanthoxylum coco Against BRCA1-Deficient Cancer Cells. Frontiers in Pharmacology, 11, 593845.

Publication 2020

Odyssey CLX System α-phospho-Akt (Ser473; anti-β-Actin goat α-mouse IRDye 680RD goat α-rabbit IRDye 800CW

Actin Antibodies Goat Irdye 800cw Mouse Rabbit Western blot analysis

Corresponding Organization : Instituto Multidisciplinario de Biología Vegetal

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Phosphorylation of Akt (Ser473)
Abundance of β-Actin

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!