Immunohistochemistry was performed as described previously 20 (link). Briefly, deparaffinized sections were subject to antigen retrieval, followed by treatment with 10% H2O2/methanol for 10 minutes to quench the endogenous peroxidase activity. The sections were incubated with primary antibodies at 4°C overnight. The primary antibodies used in this research are as follows: β-Catenin (ab22656, abcam); Axin2 (ab109307, abcam).
Protocol detail
Immunohistochemistry for β-Catenin and Axin2
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Corresponding Organization : Capital Medical University
Other organizations : Central South University, Xiangya Hospital Central South University, Dalian Medical University
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Variable analysis
independent variables
- Primary antibodies used: β-Catenin (ab22656, abcam); Axin2 (ab109307, abcam)
- Immunohistochemistry data
- Deparaffinized sections
- Antigen retrieval
- Quenching of endogenous peroxidase activity with 10% H2O2/methanol for 10 minutes
- No positive or negative controls explicitly mentioned
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