HEK293T cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Sigma-Aldrich), human lung carcinoma cells (A549) were cultured in F12K medium (Life Technologies) supplemented with 10% FBS, and Madin-Darby canine kidney (MDCK) cells were cultured in MEM (Life Technologies) containing 5% newborn calf serum (Sigma-Aldrich). All cells were maintained in a humidified incubator containing 5% CO2 at 37°C.
A/chicken/Vietnam-Ca Mau/1180/2006 (VN/1180, H5N1) and A/Anhui/1/2013 (AH/1, H7N9) were grown in 10-day-old specific-pathogen-free (SPF) embryonated chicken eggs, as reported previously [6 (link),42 (link)]. A/WSN/33 (WSN, H1N1) and a 2009 pandemic H1N1 virus, A/Fuzhou/1/2009 (FZ/1, H1N1), were propagated in MDCK cells cultured in MEM containing 0.3% bovine serum albumin (BSA, Sigma-Aldrich) and 0.5 μg/ml N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich).
A/chicken/Vietnam-Ca Mau/1180/2006 (VN/1180, H5N1) and A/Anhui/1/2013 (AH/1, H7N9) were grown in 10-day-old specific-pathogen-free (SPF) embryonated chicken eggs, as reported previously [6 (link),42 (link)]. A/WSN/33 (WSN, H1N1) and a 2009 pandemic H1N1 virus, A/Fuzhou/1/2009 (FZ/1, H1N1), were propagated in MDCK cells cultured in MEM containing 0.3% bovine serum albumin (BSA, Sigma-Aldrich) and 0.5 μg/ml N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Sigma-Aldrich).
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