Assessing Sperm Morphology Abnormalities

For sperm morphology, 200 spermatozoa per replicate per animal were assessed to determine their normality. Smears were stained with Eosin Y (Leica Biosystems Richmond Inc., Buffalo Grove, IL, USA) and spermatozoa with the following morphologies were considered to be abnormal: (i) headless, (ii) hookless, (iii) double headed, (iv) broken tail, (v) coiled tail, and (vi) double tailed.27 (link)28

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Abdullah F., Nor-Ashikin M.N., Agarwal R., Kamsani Y.S., Abd Malek M., Bakar N.S., Mohammad Kamal A.A., Sarbandi M.S., Abdul Rahman N.S, & Musa N.H. (2021). Glutathione (GSH) improves sperm quality and testicular morphology in streptozotocin-induced diabetic mice. Asian Journal of Andrology, 23(3), 281-287.

Publication 2021

Eosin Y

Animal Buffalo Eosin y Replicate Spermatozoa

Corresponding Organization : Hospital Sungai Buloh

Other organizations : International Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Percentage of spermatozoa with normal morphology

control variables

Number of spermatozoa assessed per replicate per animal (200)
Staining method (Eosin Y)
Morphological criteria for abnormal spermatozoa (headless, hookless, double headed, broken tail, coiled tail, double tailed)

controls

None explicitly mentioned

Annotations

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