Cell lines were authenticated (most recent date listed [ ]) by the University of Arizona Genetics Core and mycoplasma tested (Lonza #LT07–418). Lab stocks were made following authentication and used for this study. MCF-7 (ATCC; DMEM+10% FBS [06/29/16]), T47D (ATCC; RPMI+10% FBS [02/08/17]), ZR75.1 (ATCC; RPMI+10% FBS [10/13/16]), MDA-MB-231 (ATCC; DMEM+10% FBS [10/13/16]), MDA-MB-134-VI (ATCC; 50/50 DMEM/L15+10% FBS [02/08/17]), SUM44PE (Asterand; DMEM/F12+2% CSS with 5ug/ml insulin, 1ug/ml hydrocortisone, 5mM ethanolamine, 5ug/ml transferrin, 10nM triodothyronime, and 50nM sodium selenite [02/08/17 – no reference profile exists in database]), and BCK426 (link) (MEM+5% FBS with 1nM insulin and 1× NEAA [10/13/16 – no reference profile exists in database) cells were cultured with indicated media conditions.
Protocol detail
Authentication and Mycoplasma Testing of Cell Lines
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Cell lines
Cells
Conditions cultured media
Ethanolamine
Hydrocortisone
Insulin
Mycoplasma
Sodium selenite
Transferrin
Corresponding Organization : Cancer Research Center
Other organizations : Baylor College of Medicine, University of Colorado Anschutz Medical Campus
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Variable analysis
independent variables
- Cell lines used: MCF-7, T47D, ZR75.1, MDA-MB-231, MDA-MB-134-VI, SUM44PE, BCK4
- Not explicitly mentioned
- Authentication and mycoplasma testing of cell lines
- Culture media conditions for each cell line
- Positive control: None mentioned
- Negative control: None mentioned
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