Tumor Targeting with 64Cu-Labeled Antibody

PET imaging was performed four weeks after tumor inoculation with a method described previously.^{20 (link), 22 (link)} Briefly, each mouse was given 3.7 MBq of ⁶⁴Cu-DOTA-Pab-IR800 or ⁶⁴Cu-DOTA-IgG via the tail vein injection. The PET images were acquired at 4, 24, and 48 h post injection using a small animal PET/CT system (GE eXplore Vista) while the mice were under isoflurane-induced anesthesia. The regions of interest (ROIs) were calculated to percentage injected dose per gram tissue (%ID/g) based on the assumption of 1g/mL tissue density. Biodistribution study was performed in nude mice bearing NCI/ADR-RES tumors. Mice were given intravenously approximate 5.5 MBq of ⁶⁴Cu-DOTA-Pab-IR800 and ⁶⁴Cu-DOTA-IgG, and were sacrificed 48h post injection. Tissues and organs of interest were excised and weighed. Radioactivity was measured by a γ-counter (PerkinElmer) and the tracer uptake was calculated as %ID/g.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Wang M., Mao C., Wang H., Ling X., Wu Z., Li Z, & Ming X. (2017). Molecular Imaging of P-glycoprotein in Chemoresistant Tumors using a Dual-Modality PET/Fluorescence Probe. Molecular pharmaceutics, 14(10), 3391-3398.

Publication 2017

eXplore Vista γ-counter

Anesthesia Animal Dota Isoflurane Mice Nude mice Pet ct Radioactivity Tail Tissues Tumor inoculation Tumors Vein

Corresponding Organization :

Other organizations : University of North Carolina at Chapel Hill, Imaging Center, Wake Forest University

Top 5 similar protocols

Variable analysis

independent variables

Time post injection (4 h, 24 h, 48 h)

dependent variables

Tracer uptake (%ID/g) in tissues and organs
PET image intensity

control variables

Mouse model (nude mice bearing NCI/ADR-RES tumors)
Tracer administration (intravenous injection of approximately 5.5 MBq of 64Cu-DOTA-Pab-IR800 and 64Cu-DOTA-IgG)
Imaging technique (PET/CT system)
Anesthesia (isoflurane)

controls

Positive control: 64Cu-DOTA-Pab-IR800
Negative control: 64Cu-DOTA-IgG

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!