For annexin V/propidium iodide (PI) staining the FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen, San Diego, CA) was used according to the manufacturer's instructions. Staining for PI was performed as previously described [51 (link)]. The data were analysed with the FlowJo software (version 8.7.1; Tree Star, Ashland, OR, U.S.A.).
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