Control non-silencing siRNA was from Qiagen. Human-specific Notch1 siRNA pool was from Dharmacon and panMena and MenaINV siRNA from Ambion1 (link). A total of 1 × 106 MDA-MB-231 cells were transfected with 2 μm siRNA using the Lonza Nucleofection Kit V 72 h before each experiment. Immunoblot analysis and/or qPCR were performed to confirm knockdown for each experiment.
Free full text: Click here