Quantification of Plant Hormones in Berries

For the quantification of auxin, cytokinin, gibberellin and brassinosteroid, 1 g of fresh berries was ground in a mortar and homogenized in extraction solution (9 ml of PBS, pH 7.2–7.4) for enzyme-linked immunosorbent assay (ELISA) [49 (link)]. The ELISA procedures were conducted according to the instructions provided by the manufacturer (Enzyme-linked biological technology Co., LTD., Shanghai, China) on the Thermo Labsystems AC8 and Labsystems Multiskan MS 352 (Thermo Labsystems Co., Vantaa, Finland).

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Leng F., Cao J., Wang S., Jiang L., Li X, & Sun C. (2018). Transcriptomic Analyses of Root Restriction Effects on Phytohormone Content and Signal Transduction during Grape Berry Development and Ripening. International Journal of Molecular Sciences, 19(8), 2300.

Publication 2018

Labsystems Multiskan MS 352

Auxin Berries Biological Brassinosteroid Cytokinin Enzyme Enzyme linked immunosorbent assay Gibberellin

Corresponding Organization : Zhejiang University

Other organizations : Yangzhou University, Shanghai Jiao Tong University

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Variable analysis

independent variables

Extraction solution (9 ml of PBS, pH 7.2–7.4)

dependent variables

Quantification of auxin, cytokinin, gibberellin and brassinosteroid

control variables

Fresh berries (1 g)

controls

No positive or negative controls were explicitly mentioned.

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