All MRI studies were performed in the UCD Small Animal Imaging Facility. All animals underwent MRI imaging at 72 hours, one week, one month and two months after injury, using pre- and post-gadolinium-enhanced (0.2 mmol/kg Omniscan® IV) T1-weighted, T2-weighted, and diffusion-weighted sequences. For all MRIs, the rats were anesthetized with 2.5% isoflurane. Scans were performed on a 4.7 Tesla Bruker PharmaScan. A quadrature birdcage coil (inner diameter 38 mm, so-called “rat-brain-coil”), tuned to the 1H frequency of 200.27 MHz, was used for RF transmission and reception. T2-weighted MRI (to visualize and confirm injury) was acquired using a rapid acquisition with relaxation enhancement (RARE, Bruker manufacturer label for a fast spin echo sequence) protocol with following parameters: Field of view (FOV) = 4.6 cm; echo time/repetition time (TE/TR) = 100/4,000 msec; slice thickness = 1.20mm; no interslice gaps; number of slices = 18; number of averages = 8; matrix size = 128×256; total acquisition time = 8 min 31 sec. T1-weighted MR images (for volumetric measurements and BBBD assessment) were acquired using a multi-slice multi-echo (MSME, Bruker manufacturer label for a spin echo sequence, in this case with one echo) sequence, before and 5 minutes after administration of 0.2 mmol/kg Omniscan® via tail vein. The following acquisition parameters were used: FOV = 4.6 cm TE/TR = 11/900 msec; slice thickness =1.20 mm with no gaps applied; number of slices = 18; number of averages = 2; matrix size = 128×256; total acquisition time = 3 min 50 sec. Finally, the DWI protocol (for water diffusity/edema assessment) with 6 b-values was acquired using echo-planar imaging with the following parameters: FOV = 4.6 cm; TE/TR = 40/3,000 msec; slice thickness =2 mm with an interslice gap = 0.5 mm between the slices; number of slices = 4; number of averages = 16; b-values = 0, 150, 300, 600, 800 and 1000 sec/mm2 in the x-direction; matrix size = 64×64; total acquisition time = 4 min 48 sec. All images were acquired in the axial orientation.
Protocol detail
Multimodal MRI of Traumatic Brain Injury
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Animals
Brain
Diffusion
Echo
Edema
Gadolinium
Injury
Isoflurane
Omniscan
Scans
Spin label
Tail
Transmission
Vein
Corresponding Organization : Colorado State University
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Protocol cited in 6 other protocols
Variable analysis
independent variables
- Time points of MRI imaging (72 hours, 1 week, 1 month, 2 months after injury)
- T1-weighted MRI (for volumetric measurements and BBBD assessment)
- T2-weighted MRI (to visualize and confirm injury)
- Diffusion-weighted MRI (for water diffusity/edema assessment)
- All MRI studies were performed in the UCD Small Animal Imaging Facility
- All animals underwent MRI imaging using pre- and post-gadolinium-enhanced (0.2 mmol/kg Omniscan® IV) T1-weighted, T2-weighted, and diffusion-weighted sequences
- For all MRIs, the rats were anesthetized with 2.5% isoflurane
- Scans were performed on a 4.7 Tesla Bruker PharmaScan
- A quadrature birdcage coil (inner diameter 38 mm, so-called "rat-brain-coil"), tuned to the ^1H frequency of 200.27 MHz, was used for RF transmission and reception
- All images were acquired in the axial orientation
Annotations
Based on most similar protocols
Optimize the anesthesia dosage and monitor the animal's vital signs (e.g., respiration rate, body temperature) during the MRI scans to ensure stable physiological conditions (Protocol 4).
Use a fast shimming procedure (Fastmap) to improve the B0 homogeneity covering the brain, which is critical for high-quality MRI and spectroscopy data (Protocol 4).
Include a reference standard, such as a spherical quality assurance phantom, to validate the MRI and spectroscopy data (e.g., achieve a full width half maximum (FWHW) of 5 Hz for the unsuppressed water peak in the spectroscopy voxel) (Protocol 4).
Acquire both proton density (PD) and T2-weighted images in the axial and coronal planes to provide comprehensive structural information about the brain (Protocol 4).
Obtain diffusion-weighted images with multiple b-values (e.g., 1000, 1500, and 2000 s/mm^2) and gradient directions (e.g., 30) to enable more advanced diffusion tensor imaging (DTI) and diffusion kurtosis imaging (DKI) analyses (Protocol 4).
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