Dark-adapted ERGs were used to assess inner and outer retinal neuronal function (see Supplementary Materials). ERG measurements were taken at baseline (before the crosslinking procedure), and at 7 and 14 days after microbead injection (Fig. 1C). We analyzed amplitudes of the positive scotopic threshold and negative scotopic threshold signals at –6.0 log cd s/m2, the b-wave at –3.0 log cd s/m2 (b-wave), and the third oscillatory potential (OP3) at 2.1 log cd s/m2, because these amplitudes have been shown to be significantly decreased in rodent models of glaucoma.35 (link)39 (link)
Visual function was assessed via quantitative analysis of OMR thresholds of spatial frequency and contrast sensitivity (CS) (see Supplementary Materials, OptoMotry; Cerebral-Mechanics, Lethbridge, AB, Canada). The OMR was measured at baseline (before crosslinking treatment), day 0 (7 days after crosslinking procedure, but before microbead injection that day), day 7, and day 14 (Fig. 1C), following a protocol similar to that of Prusky et al. and Douglas et al.40 (link),41 (link)