Evaluating Retinal Function in Glaucoma Mouse Model

Dark-adapted ERGs were used to assess inner and outer retinal neuronal function (see Supplementary Materials). ERG measurements were taken at baseline (before the crosslinking procedure), and at 7 and 14 days after microbead injection (Fig. 1C). We analyzed amplitudes of the positive scotopic threshold and negative scotopic threshold signals at –6.0 log cd s/m², the b-wave at –3.0 log cd s/m² (b-wave), and the third oscillatory potential (OP3) at 2.1 log cd s/m², because these amplitudes have been shown to be significantly decreased in rodent models of glaucoma.³⁵ (link)^–39 (link)
Visual function was assessed via quantitative analysis of OMR thresholds of spatial frequency and contrast sensitivity (CS) (see Supplementary Materials, OptoMotry; Cerebral-Mechanics, Lethbridge, AB, Canada). The OMR was measured at baseline (before crosslinking treatment), day 0 (7 days after crosslinking procedure, but before microbead injection that day), day 7, and day 14 (Fig. 1C), following a protocol similar to that of Prusky et al. and Douglas et al.⁴⁰ (link)^,41 (link)