Isolation and Characterization of MDSCs and DCs

MDSC were isolated from splenocytes by positive selection with FITC anti-Ly6C, PE anti-Ly6G, or PE anti-Gr1 using anti-FITC or anti-PE microbeads (Miltenyi Biotec), as described (12 , 14 (link)). DC were isolated by CD11c immunomagnetic bead selection and γ-irradiated (20 Gy). T cell proliferation was assessed at 72 h by [³H] TdR incorporation or CFSE dilution (Invitrogen).

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Rosborough B.R., Mathews L.R., Matta B.M., Liu Q., Raïch-Regué D., Thomson A.W, & Turnquist H.R. (2014). Flt3 ligand mediates STAT3-independent expansion, but STAT3-dependent activation of myeloid-derived suppressor cells. Journal of immunology (Baltimore, Md. : 1950), 192(8), 3470-3473.

Publication 2014

CFSE dilution

Cell proliferation Cfse Dilution Fitc Mdsc Microbeads

Corresponding Organization : University of Pittsburgh

Other organizations : Harbin Medical University, Second Affiliated Hospital of Harbin Medical University

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Variable analysis

independent variables

MDSC isolation by positive selection with FITC anti-Ly6C, PE anti-Ly6G, or PE anti-Gr1 using anti-FITC or anti-PE microbeads
DC isolation by CD11c immunomagnetic bead selection
γ-irradiation of DC (20 Gy)

dependent variables

T cell proliferation assessed at 72 h by [3H] TdR incorporation or CFSE dilution

control variables

Splenocytes used as the source of MDSC and DC

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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