Measuring Nitric Oxide Production

Perfused CAs and Affs were loaded with 5 × 10⁻⁵ mol ∙ L⁻¹ 4-amino-5-methylamino -2’-7’ difluorescein diacetate (DAF-FM-DA; Sigma-Aldrich, St Louis, MO 63103). Increased fluorescence after 1 minute of Ang II (10⁻⁶ mol ∙ L⁻¹) or BW245c (10⁻⁵ mol ∙ L⁻¹) vs vehicle quantitated NO production.^{37 (link)}

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Li L., Lai E., Cao X., Welch W, & Wilcox C. (2020). Endothelial prostaglandin D2 opposes angiotensin II contractions in mouse isolated perfused intracerebral microarterioles. Journal of the Renin-Angiotensin-Aldosterone System: JRAAS, 21(4), 1470320320966177.

Publication 2020

DAF-FM-DA

Ang 1 Fluorescence

Corresponding Organization : Georgetown University

Other organizations : Zhejiang University

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Variable analysis

independent variables

Angiotensin II (Ang II) at 10^-6 mol · L^-1
BW245c at 10^-5 mol · L^-1

dependent variables

Nitric oxide (NO) production quantitated by increased fluorescence after 1 minute

control variables

Perfused CAs and Affs loaded with 5 × 10^-5 mol · L^-1 4-amino-5-methylamino -2'-7' difluorescein diacetate (DAF-FM-DA)

controls

Vehicle (control condition)

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