Total RNA was extracted from BRCA cell lines using the TRIzol reagent (Thermo Fisher Scientific, Inc.) and was transcribed to cDNA using the RevertAid First Strand cDNA Synthesis kit (Thermo Fisher Scientific, Inc.). Reverse transcription was conducted at 37°C for 1 h, followed by 85°C for 5 sec according to the manufacturer's protocol. RT-qPCR was performed using the QuantiTect SYBR-Green PCR kit (Roche Diagnostics) as previously described (30 (link)). The primer sequences are listed in Table SI. The 2−ΔΔCq method was used to calculate the relative expression levels of the target genes (31 (link)). GAPDH was used for normalization. The reaction conditions were as follows: 95°C for 10 min, followed by 95°C for 30 sec, 60°C for 30 sec and 72°C for 30 sec (39 cycles), and finally 72°C for 10 min.