Mouse lymphoma cell lines, RMA (H-2b) and TAP-2-deficient RMA-S (H-2b),19 (link) were cultured in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO) with 10% FCS (Sigma-Aldrich) (R-10). The HHD gene-transfected cell lines, RMA-HHD and RMA-S-HHD, were previously described,15 (link) and maintained in R-10 containing 500 μg/ml G418 (Nacalai Tesque, Kyoto, Japan). A human kidney cell line, 293 cell lines were obtained from the American Type Culture Collection (ATCC) (Rockville, MD). The Ad-293 cell line was provided with the AdEasy adenoviral vector system (Agilent Technologies, La Jolla, CA). 293 and Ad-293 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10% FCS (Sigma-Aldrich).
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Mouse lymphoma cell lines protocol
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Corresponding Organization : Saitama Medical University
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Variable analysis
independent variables
- Cell lines: RMA (H-2^b), TAP-2-deficient RMA-S (H-2^b), RMA-HHD, RMA-S-HHD, 293, and Ad-293
- Not explicitly mentioned
- Culture medium: RPMI-1640 with 10% FCS (R-10) for RMA, RMA-S, RMA-HHD, and RMA-S-HHD cell lines
- Culture medium: DMEM with 10% FCS for 293 and Ad-293 cell lines
- G418 (500 μg/ml) for maintaining RMA-HHD and RMA-S-HHD cell lines
- Not explicitly mentioned
- Not explicitly mentioned
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