Antioxidant Analysis in Mice Jejunum
Corresponding Organization : Applied Science Private University
Other organizations : King Saud University, University of Hafr Al-Batin, Princess Nourah bint Abdulrahman University
Variable analysis
- Experimental groups
- Weight of mice jejuna
- Reduced glutathione (GSH) concentration
- Glutathione peroxidase (GPx) level
- Malondialdehyde (MDA) contents
- Nitric oxide (NO) level
- Superoxide dismutase (SOD) quantity
- Homogenization in 300 mM sucrose and 50 mM Tris(hydroxymethyl)aminomethane-HCl solution
- Centrifugation at 500×g at 4 °C for 10 min
- Use of proper kits (Biodiagnostic Co., Egypt) and SoftMax® Pro v. 6.3.1 software
- Molecular device used for analysis: Spectra MAX 190
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