Standardized Blood Sampling and Analysis

Blood was collected by qualified research assistants using a standardized protocol (Supplementary Data). Blood for chemistry and hematology measurements was analyzed by the UMCG. Plasma glucose concentration was measured on an YSI 2300 Stat glucose/lactate analyzer (YSI, Inc., Yellow Springs, OH). Serum insulin concentration was measured using radioimmunoassay (Diagnostic Laboratories, Los Angeles, CA) (Cree et al, 2008 (link)). Total GLP-1, GLP-2, and ghrelin were analyzed at DSM Nutritional Products, Switzerland, by using commercially available ELISA kits (Millipore Corp).

Free full text: Click here

Liu L., Sadaghian Sadabad M., Gabarrini G., Lisotto P., von Martels J.Z., Wardill H.R., Dijkstra G., Steinert R.E, & Harmsen H.J. (2023). Riboflavin Supplementation Promotes Butyrate Production in the Absence of Gross Compositional Changes in the Gut Microbiota. Antioxidants & Redox Signaling, 38(4), 282-297.

Publication 2023

Blood Blood chemistry Diagnostic Elisa Ghrelin Glp 1 Glucose Insulin Lactate Plasma Radioimmunoassay Serum Springs

Corresponding Organization : University of Groningen

Other organizations : South Australian Health and Medical Research Institute, University of Adelaide, DSM (Switzerland), University Hospital of Zurich

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma glucose concentration
Serum insulin concentration
Total GLP-1
GLP-2
Ghrelin

control variables

Blood was collected using a standardized protocol

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!