Quantifying Glandular Trichome Density

To assess GST density, mature leaves (leaf 9) from transgenic plants T0 and T1 with increased artemisinin content and wild-type A. annua were collected. Images from the adaxial side of the leaves were taken using fluorescence microscopy (Olympus BX51, Tokyo, Japan) equipped with an A×5 objective lens. The fluorescence excitation was captured at 450–480 nm, and the ImageJ software (http://rsb.info.nih.gov/ij) was used to measure the leaf area and count the number of GSTs, as previously described (Cheng et al., 2014 (link)).

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Hassani D., Taheri A., Fu X., Qin W., Hang L., Ma Y, & Tang K. (2023). Elevation of artemisinin content by co-transformation of artemisinin biosynthetic pathway genes and trichome-specific transcription factors in Artemisia annua. Frontiers in Plant Science, 14, 1118082.

Publication 2023

Artemisinin Fluorescence Fluorescence microscopy Gsts Leaf Lens Transgenic plants

Corresponding Organization : Shanghai Jiao Tong University

Other organizations : East China Normal University

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Variable analysis

independent variables

Transgenic plants (T0 and T1) with increased artemisinin content
Wild-type Artemisia annua

dependent variables

GST density

control variables

Mature leaves (leaf 9) were collected
Images were taken from the adaxial side of the leaves
Fluorescence microscopy (Olympus BX51, Tokyo, Japan) with A×5 objective lens was used
Fluorescence excitation was captured at 450–480 nm
ImageJ software was used to measure leaf area and count the number of GSTs

controls

No positive or negative controls were explicitly mentioned in the protocol.

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