The assay was carried out according to the method described earlier (50 (link)). The reaction mixture contained 50 mM Tris–HCl, 0.1 M potassium acetate buffer (pH 8.0), 0.1–5 mM FBP, 0.2 mM NADH, and a mixture of coupling enzymes, glycerol phosphate dehydrogenase, and triosephosphate isomerase. The reaction was initiated by the addition of retina lysate and we monitored the decrease in the absorbance at 340 nm from the oxidation of NADH.
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