Freshly frozen liver tissues were homogenized and centrifuged, and the supernatants were collected [27 (link)]. The plasma and hepatic concentrations of TNF-α, IL-6, leptin, and adiponectin were then assayed (TNF-α, IL-6, leptin, and adiponectin enzyme-linked immunosorbent assay kits; all from Enzo Life Science, Farmingdale, NY, USA).
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