Cells were cultured in growth medium and 50 ng/mL Nocodazole were added 16 h prior to fixation. Metaphase spreads were prepared as previously described. Telomere and centromeres were concomitantly hybridized with TelC-Cy3 (PNA Bio) and CENPB-Cy5 (PNA Bio) for 2 h at RT after denaturation at 75 °C. After dehydration, slides were stained with 1 µg/mL DAPI (KPL) and mounted with ProLong Gold before imaging.
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