Brucella Isolation from Wild Boar Tissues

For the bacteriological investigation, tissue samples of wild boars, including testicles, uterus, lymph nodes, and spleen were dissected under sterile conditions and plated on selective Brucella agar (Oxoid, Wesel, Germany). Besides the nutritional ingredients, this medium contains a supplement cocktail. Due to the slow growth of Brucella species, effective suppression of other bacteria and fungi needs to be achieved during incubation. The substances polymyxin B, bacitracin, nalidixic acid, and vancomycin are summarized as antimicrobials. The substance nystatin has an antifungal effect. Cultural incubation was performed in a microaerophilic atmosphere at 37 °C and 10% CO₂ for five to seven days [19 ,20 ]. To meet biosafety standards, suspicious Brucella colonies were transferred into a BSL-3 laboratory, confirmed by MALDI-TOF mass spectrometry (Bruker, Bremen, Germany), and preserved at −80 °C until further investigation.