All experimental procedures were conducted in accordance with EU regulations in the Directive 2010/63/EU of the European Parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes. We have ethical approval from the national Bulgarian Food Safety Agency, Ministry of Agricultural, Food and Forestry (No. 224 from 23 January 2019) and from the local Ethics Committee of Faculty of Biology, Sofia University “St. Kliment Ohridski” (Protocol No. 2/2022). All efforts were made to minimize the number of animals used and their suffering.
The livers for assay of SSAO activity, intact liver mitochondria, and SMPs were isolated from mature male Wistar rats (50–60 days old, supplied by Vivarium with Physiological Laboratory, Project: Creation and Development of Centers of Competence: BG05M2OP001-1.002-0012-C01, “Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products”). The animals were housed in cages at 20–24 °C for 12 h light/dark cycle. They received a standard laboratory diet (commercial rat chow, HL-TopMix Ltd., Kaloyanovo, Bulgaria) and water ad libitum and were fasted prior to use. Rats were anesthetized and then decapitated, and the liver was surgically removed.
The livers for assay of SSAO activity, intact liver mitochondria, and SMPs were isolated from mature male Wistar rats (50–60 days old, supplied by Vivarium with Physiological Laboratory, Project: Creation and Development of Centers of Competence: BG05M2OP001-1.002-0012-C01, “Sustainable utilization of bio-resources and waste of medicinal and aromatic plants for innovative bioactive products”). The animals were housed in cages at 20–24 °C for 12 h light/dark cycle. They received a standard laboratory diet (commercial rat chow, HL-TopMix Ltd., Kaloyanovo, Bulgaria) and water ad libitum and were fasted prior to use. Rats were anesthetized and then decapitated, and the liver was surgically removed.
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