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Flow Cytometric Analysis of T Cells

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Analyses of CD4+ and CD8+ T lymphocytes were performed according to our previous study [25 (link)]. The percentages of CD4+ and CD8+ T lymphocytes were determined using flow cytometry with staining by the surface markers including phycoerythrin- (PE-) labeled anti-mouse CD3 (eBioscience), allophycocyanin- (APC-) labeled anti-mouse CD4 (eBioscience), and fluorescein isothiocyanate- (FITC-) labeled anti-mouse CD8 (eBioscience) antibodies. All the samples were analyzed regarding fluorescence profiles on a FACScan flow cytometer (BD Biosciences) by SYSTEM II software (Coulter).

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Hu L.Y., Zhang N.Z., Zhang F.K., Wang M., Gao Q., Wang J.L, & Zhu X.Q. (2017). Resistance to Chronic Toxoplasma gondii Infection Induced by a DNA Vaccine Expressing GRA16. BioMed Research International, 2017, 1295038.

Publication 2017

PE-) labeled anti-mouse CD3 allophycocyanin- (APC-) labeled anti-mouse CD4 fluorescein isothiocyanate- (FITC-) labeled anti-mouse CD8 FACScan flow cytometer SYSTEM II software

Allophycocyanin Anti cd3 Antibodies Cd8 t lymphocytes Fitc Flow cytometry Fluorescein Fluorescence Isothiocyanate Mouse Phycoerythrin

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Percentage of CD4+ T lymphocytes
Percentage of CD8+ T lymphocytes

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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