Monoclonal Antibodies for SFTS Virus

Anti-feline CD2 monoclonal antibody SKR2 and anti-SFTS virus nuclear protein monoclonal antibody 9D3 were reported previously^{57 (link),58 (link)}. Purified SFTS virus-neutralising antibody was prepared from sera of monkeys that were inoculated with SFTS virus using Protein G Sepharose 4 Fast Flow (GE Healthcare) (Shimojima et al., manuscript in preparation). Mouse anti-SFTS virus GP monoclonal antibodies (Immune Technology Corp., clones 62D6 and 21D2), mouse IgG1 isotype control (R&D Systems), mouse anti-DC-SIGN/DC-SIGNR (clone DC28, R&D Systems), mouse anti-LSECtin monoclonal antibody (clone SOTO-1, Santa Cruz Biotechnology), normal human IgG (Wako Pure Chemical Industries, Ltd.), goat anti-mouse IgG (H + L) Alexa Fluor488 (Thermo Fisher Scientific), 2′-deoxy-2′-fluorocytidine hydrate (Tokyo Chemical Industry Co., Ltd.), N-butyldeoxynojirimycin (Cayman Chemical), WST-1 (Sigma-Aldrich) and 1 kb DNA Ladder (New England Biolabs Japan) were purchased.

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Shimojima M., Sugimoto S., Taniguchi S., Yoshikawa T., Kurosu T, & Saijo M. (2020). Efficient functional screening of a cellular cDNA library to identify severe fever with thrombocytopenia syndrome virus entry factors. Scientific Reports, 10, 5996.

Publication 2020

Protein G Sepharose 4 Fast Flow mouse IgG1 isotype control normal human IgG goat anti-mouse IgG (H + L) Alexa Fluor488 WST-1 1 kb DNA Ladder

2 deoxy 2 fluorocytidine Anti antibodies Anti antibody Anti cd2 monoclonal antibody Anti igg Anti nuclear antibody Cayman Clone Dc sign Feline Goat Human Igg1 Isotype Monkeys Monoclonal antibody Mouse N butyldeoxynojirimycin Neutralising antibody Protein g Protein monoclonal Sepharose Sera Sfts virus Skr2

Corresponding Organization : National Institute of Infectious Diseases

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Variable analysis

independent variables

Anti-feline CD2 monoclonal antibody SKR2
Anti-SFTS virus nuclear protein monoclonal antibody 9D3
Purified SFTS virus-neutralising antibody prepared from sera of monkeys inoculated with SFTS virus
Mouse anti-SFTS virus GP monoclonal antibodies (clones 62D6 and 21D2)
2′-deoxy-2′-fluorocytidine hydrate
N-butyldeoxynojirimycin

dependent variables

Neutralizing activity of purified SFTS virus-neutralising antibody

control variables

Mouse IgG1 isotype control
Mouse anti-DC-SIGN/DC-SIGNR (clone DC28)
Mouse anti-LSECtin monoclonal antibody (clone SOTO-1)
Normal human IgG
Goat anti-mouse IgG (H + L) Alexa Fluor488
WST-1
1 kb DNA Ladder

controls

Positive control: Not specified
Negative control: Mouse IgG1 isotype control, normal human IgG

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