Isolation and Characterization of Monoterpene Biosynthesis Regulatory Fragments

Upstream regulatory fragments of three monoterpene biosynthesis genes, including PbGDPS, PbTPS5, and PbTPS10, were isolated from P. bellina genomic DNA by use of the Universal GenomeWalker Kit (Clontech, USA) as previously described (Hsu et al. 2014 (link)). In addition, we also isolated 1.5-kb upstream regulatory fragments of the identified six TFs involved in the regulation of floral monoterpene biosynthesis in P. bellina (Chuang et al. unpublished) from P. equestris genomic sequences (Cai et al. 2015 (link)). The cis-elements related to light signaling and circadian clock on the upstream regulatory fragments of both monoterpene biosynthesis genes and TFs were predicted by using PlantPAN (Chow et al. 2015 (link)). The predicted results with 100% similar score were accepted.

Free full text: Click here

Chuang Y.C., Lee M.C., Chang Y.L., Chen W.H, & Chen H.H. (2017). Diurnal regulation of the floral scent emission by light and circadian rhythm in the Phalaenopsis orchids. Botanical Studies, 58, 50.

Publication 2017

Universal GenomeWalker Kit

Biosynthesis Circadian clock genes Genomic Monoterpene Regulatory genes

Corresponding Organization :

Other organizations : National Cheng Kung University

Top 5 similar protocols

Variable analysis

independent variables

Upstream regulatory fragments of three monoterpene biosynthesis genes, including PbGDPS, PbTPS5, and PbTPS10, isolated from P. bellina genomic DNA
1.5-kb upstream regulatory fragments of the identified six TFs involved in the regulation of floral monoterpene biosynthesis in P. bellina isolated from P. equestris genomic sequences

dependent variables

Cis-elements related to light signaling and circadian clock on the upstream regulatory fragments of both monoterpene biosynthesis genes and TFs

control variables

Predicted results with 100% similar score were accepted

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!