The phosphorylation of TFAM was assayed using the immunoprecipitation assay. Cells were lysed and incubated with 200 μL Protein A beads (Santa Cruz Biotechnology) supplemented with 50 μg anti-TFAM antibody overnight. Normalized amounts of total lysates or immunoprecipitated samples were analyzed by SDS-PAGE and western blotting with phosphorylated antibody.19 (link)
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