Real-time PCR analysis was performed as previously described [24 (link),27 (link)]. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was utilized in experiments as a control gene [24 (link)]. We purchased the primers and probes for mouse Osterix, Osteocalcin, Osteopontin, Foxc2, Flk1, VE-cadherin, and CD31 from Applied Biosystems (Waltham, MA, USA) as part of Taqman® Gene Expression Assays.
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