Immunosuppressive Pathway Targeting Protocol

N₃-PEG₂₀₀₀-ACA was purchased from MeloPEG (Shengzhen, China). 1,4-butanediol diacrylate (HDD), 4,4’-trimethyldipiperidine (TDP), tris (3-hydroxypropyltriazolyl methyl) amine (THPTA), fructose-6-phosphate (F6P), IR-780 were purchased from Aladdin (Shanghai, China). BAY-876 and UDP-GlcNAc were purchased from MedChemExpress (Shanghai, China). Annexin V-FITC apoptosis detection kit, DAPI, and TUNEL detection kit were purchased from Beyotime (Shanghai, China). ELISA kit of IFN-γ, TNF-α, CXCL-10 and granzyme were purchased from ABclonal (Wuhan, China). Fructose-6-Phosphate (F6P) Assay Kit was purchased from Sigma-Aldrich. Red blood cell lysis buffer and Glycerol anhydrous were obtained from Solarbio (Beijing, China). Mouse-PD1 protein, Mouse-biotin-PD-L1 and Mouse-biotin-CTLA-4 were purchased from ACROBiosystems (Beijing, China). Streptavidin-coated magnetic beads were purchased from BEAVER (Suzhou, China). DNA was purchased from Sangon (Shanghai, China), and the corresponding sequence information was provided in Supplementary Table 1. siNC and siPD-L1 were purchased from Biosyntech (Suzhou, China), and the corresponding sequence information was provided in Supplementary Table 2. The information of antibodies was provided in Supplementary Tables 3 and  4.