Comprehensive Analysis of Cancer Cell Properties

Total cell number counting, BrdU incorporation, cell cycle analysis, TUNEL, Annexin V, foci formation, soft agar colony formation assay, 3D-matrigel culture, in vitro cell motility (migration, invasion and wound healing) assays and tumorsphere formation assay were carried out as previously described [55 (link)–57 (link)]. All the images were taken using a phase microscope (Olympus, Tokyo, Japan). The CD133 positive population in HCC cells was examined by using PE-conjugated monoclonal mouse anti-human CD133/1 (AC133, Miltenyi Biotec; Auburn CA).

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Zhang M., Zhang W., Wu Z., Liu S., Sun L., Zhong Y., Zhang X., Kong X., Qian P., Zhang H., Lobie P.E, & Zhu T. (2015). Artemin is hypoxia responsive and promotes oncogenicity and increased tumor initiating capacity in hepatocellular carcinoma. Oncotarget, 7(3), 3267-3282.

Publication 2015

phase microscope PE-conjugated monoclonal mouse anti-human CD133/1 (AC133,

Agar Annexin v Assay Brdu Cell cycle Cell motility Human Matrigel Microscope Mouse Tunel

Corresponding Organization : Hefei National Center for Physical Sciences at Nanoscale

Other organizations : Anhui Medical University, National University Cancer Institute, Singapore, National University of Singapore

Top 5 similar protocols

Variable analysis

independent variables

Total cell number counting
BrdU incorporation
Cell cycle analysis
TUNEL
Annexin V
Foci formation
Soft agar colony formation assay
3D-matrigel culture
In vitro cell motility (migration, invasion and wound healing) assays
Tumorsphere formation assay

dependent variables

Cell count
BrdU incorporation
Cell cycle distribution
TUNEL-positive cells
Annexin V-positive cells
Foci formation
Colony formation
3D-matrigel culture characteristics
Cell migration, invasion, and wound healing
Tumorsphere formation

control variables

Phase microscope (Olympus, Tokyo, Japan)
PE-conjugated monoclonal mouse anti-human CD133/1 (AC133, Miltenyi Biotec; Auburn CA)

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