Example 4
Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length by 10 mm height) were seeded with 5,000, or 10,000, or 20,000 P6 human bone marrow-derived mesenchymal stem cells per channel in 200 μl of culture media in a tissue culture dish on a rocking platform. Rocking frequency was 5 rpm and 15° tilt.
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