Quantifying Histone Acetylation by Mass Spec
Corresponding Organization : Centre Hospitalier Universitaire Sainte-Justine
Other organizations : Temple University, Coriell Institute For Medical Research, Wilkes University, Fox Chase Cancer Center, Sidney Kimmel Comprehensive Cancer Center
Variable analysis
- Acid extraction (0.2 N HCl) of histone proteins for 16 h
- Histone acetylation levels on histone 3 and histone 4
- Quantification of histone acetylation levels by a Thermo TSQ Quantum Access triple quadrupole (QqQ) mass spectrometer
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the given information.
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