As described previously [16 (link),24 (link)], histone proteins were acid-extracted (0.2 N HCl) for 16 h. Histone acetylation levels were quantified on histone 3 and histone 4 by a Thermo TSQ Quantum Access triple quadrupole (QqQ) mass spectrometer (Thermofisher Scientific, Waltham, MA, USA).
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