Total RNA was extracted from liver tissues by Trizol and complementary DNA (cDNA) was synthesized using a cDNA Reverse Transcription kit (code no. RR047A, TAKARA). Quantitative PCR was performed using the SYBR Green PCR mix (code no. RR820L, TAKARA) on a PRISM 7700 (Applied Biosystems). The specificity of the primers used (TableĀ S2) was previously confirmed54 (link),55 (link).
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