Synthesis of BKV Pseudoviruses

VP1 expression constructs used in this work were reported previously (9 (link),10 (link)). Viral isolates BKV-Ia (BK-D; accession number JF894228), BKV-Ib2 (PittVR2; DQ989796), BKV-Ic (RYU-2; AB211377), BKV-II (GBR-12; AB263920), BKV-III (KOM-3; AB211386), and BKV-IVb (THK-8; AB211390) were used to design codon-modified ORFs, which were chemically synthesized by Blue Heron Biotech. Gateway recombination technology (Invitrogen) was used to transfer the codon-modified open reading frames into expression plasmid pGwf (4 ). The codon-modified VP2/VP3 minor capsid protein genes based on BKV isolate A-66H were used for production of all BKV pseudoviruses.

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Randhawa P., Pastrana D.V., Zeng G., Huang Y., Shapiro R., Sood P., Puttarajappa C., Berger M., Hariharan S, & Buck C.B. (2015). Commercially Available Immunoglobulins Contain Virus Neutralizing Antibodies Against All Major Genotypes of Polyomavirus BK. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(4), 1014-1020.

Publication 2015

Gateway recombination technology

Capsid protein Codon Genes Open reading frames Plasmid Recombination

Corresponding Organization :

Other organizations : University of Pittsburgh, National Cancer Institute, CSL (Switzerland)

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Variable analysis

independent variables

Codon-modified ORFs of BKV isolates BKV-Ia (BK-D), BKV-Ib2 (PittVR2), BKV-Ic (RYU-2), BKV-II (GBR-12), BKV-III (KOM-3), and BKV-IV (THK-8)

dependent variables

Not explicitly mentioned

control variables

Codon-modified VP2/VP3 minor capsid protein genes based on BKV isolate A-66H used for production of all BKV pseudoviruses

controls

No positive or negative controls are explicitly mentioned

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