Two Pan-neuronal nuclear localized GCaMP6s Tg(HuC:H2B:GCaMP6s) and two pan-neuronal soma localized GCaMP7f Tg(HuC:somaGCaMP7f) [31 (link)] zebrafish larvae were imaged at 4–6 days post fertilization. Additionally, two NLS GCaMP6s fish of unknown age. The transgenic larvae were kept at and paralyzed in standard fish water containing 0.25 mg/ml of pancuronium bromide (Sigma-Aldrich) for 2 min before imaging to reduce motion. The paralyzed larvae were then embedded in agar with 0.5% agarose (SeaKem GTG) and 1% low-melting point agarose (Sigma-Aldrich) in Petri dishes.
Additionally, a beads dataset was created by imaging green fluorescent beads (ThermoFisher) randomly distributed in 1% low-melting-point agarose (Sigma-Aldrich). The stock beads were serially diluted using melted agarose to , , , of the original concentration.
Each fish was imaged for 1000 frames at 10Hz. Neural activity images were extracted using the SLNet [32 ], as seen inFig. 1(a) . Later, the resulting images were 3D reconstructed with the RL algorithm for 100 iterations, which takes roughly per frame.
Additionally, a beads dataset was created by imaging green fluorescent beads (ThermoFisher) randomly distributed in 1% low-melting-point agarose (Sigma-Aldrich). The stock beads were serially diluted using melted agarose to , , , of the original concentration.
Each fish was imaged for 1000 frames at 10Hz. Neural activity images were extracted using the SLNet [32 ], as seen in
