Chromatin Immunoprecipitation (ChIP) Protocol

ChIP assays were performed as previously described 15 (link). Briefly, cells were crosslinked with 1% formaldehyde, then glycine was used to quench the cross-linking reaction. The cells were lysed in SDS buffer supplemented with protease inhibitor cocktail. Chromatin DNA was splintered into approximately 300 base-pair fragments by ultrasonication, which were then subjected to IP with 10 μg IgG (ab172730, Abcam), or 10 μg RORα (ab256799, Abcam). Washing with low and high-salt-concentration wash buffers, the DNA fragments were de-crosslinked under high-salt conditions. The QIAquick PCR purification kit (Vazyme) was used to purify DNA, followed by qRT-PCR.