High-Resolution Ion Mobility Mass Spectrometry
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Corresponding Organization :
Other organizations : Environmental Molecular Sciences Laboratory, Battelle, Pacific Northwest National Laboratory
Protocol cited in 16 other protocols
Variable analysis
- Voltage of nanoelectrospray ionization (nanoESI) source (3000 V)
- Flow rate of sample solutions (0.3 μL/min)
- Temperature of heated stainless steel capillary (130 °C)
- Trapping time of ion funnel trap (IFT) (25 ms)
- Frequency of ion funnel trap (IFT) (950 kHz)
- Peak-to-peak voltage of ion funnel trap (IFT) (~200 V)
- Pressure in the ion funnel trap (IFT) (1.85 to 3.95 Torr)
- Release time of ions from the ion funnel trap (IFT) (486 μs)
- Offset of ion inlet capillary from the center axis of the IFT (6 mm)
- Pressure in the SLIM chamber (2–4 Torr)
- Differential positive pressure between the IFT and SLIM chamber (~50 mTorr)
- Frequency of rear ion funnel (820 kHz)
- Peak-to-peak voltage of rear ion funnel (~120 V)
- DC gradient of rear ion funnel (23 V/cm)
- Pressure in the differentially pumped region (460 mTorr)
- Frequency of RF-only quadrupole (1 MHz)
- Peak-to-peak voltage of RF-only quadrupole (~130 V)
- Ion transmission and detection by the Time-of-Flight Mass Spectrometer (TOF MS)
- Emitter inner diameter (20 μm)
- Fused-silica capillary inner diameter (30 μm)
- Stainless steel capillary inner diameter (500 μm)
- Conductance-limiting orifice diameter (2.5 mm)
- Length of converging region in the IFT (2.5 cm)
- Length of rear ion funnel (15 cm)
- Flight tube length of the TOF MS (1.5 m)
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