Genetically Modified Mouse Models

WT C57BL/6 mice and Rag2^–/– mice were obtained from Jackson Laboratories. Nr3c1^fl mice were generated by us (35 (link)) and crossed to mice expressing Foxp3 promoter–driven YFP/Cre recombinase expression for GR deletion specifically in Tregs. Cyp11b1^fl mice were generated by us (11 (link)) and crossed to mice expressing Actb or LysM promoter-driven Cre recombinase (Jackson Laboratories) expression for global or macrophage-specific Cyp11b1 deletion, respectively. We generated and characterized (Taves, unpublished observations) mice expressing an mScarlet-tagged Cyp11b1 protein (Cyp11b1^mScarlet). We did not detect any consistent sex differences in the effects of glucocorticoids on tumor growth and immune cell phenotype and therefore combined female and male mice for all analyses. Mice were kept on a 12-hour light/12-hour dark cycle, with ad libitum access to standard chow (NIH-31, Teklad).