Breast Cancer Cell Line Maintenance

The BT474 cell line was obtained from AstraZeneca (Cheshire, UK) [15 (link)]. SKBR3, MDA-MB-361, HCC1954, and HCC202 cell lines were from Dr. Joe Gray’s lab (Berkeley Lab, Berkeley, CA, USA) [16 (link), 17 (link)]. AU565, UACC812, and HCC-1569 cell lines were from ATCC. BT474, UACC-812, and MDA-MB-361 cell lines were maintained in Dulbecco’s modified Eagle medium (DMEM); SKBR3 cells were grown in McCoy’s 5A; and AU-565, HCC-1569, HCC-1954, and HCC-202 were cultured in RPMI 1640 modified with 1X L-glutamine with all media supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin-streptomycin-glutamine [17 (link), 18 (link)].

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Bhat R.R., Yadav P., Sahay D., Bhargava D.K., Creighton C.J., Yazdanfard S., Al-rawi A., Yadav V., Qin L., Nanda S., Sethunath V., Fu X., De Angelis C., Narkar V.A., Kent Osborne C., Schiff R, & Trivedi M.V. (2018). GPCRs profiling and identification of GPR110 as a potential new target in HER2+ breast cancer. Breast cancer research and treatment, 170(2), 279-292.

Publication 2018

AU565 UACC812 HCC-1569

Cell lines Cells Eagle Fetal bovine serum Glutamine Penicillin Streptomycin

Corresponding Organization : Baylor College of Medicine

Other organizations : The University of Texas Health Science Center at Houston

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Variable analysis

independent variables

Cell line

dependent variables

Not explicitly mentioned

control variables

Culture medium (DMEM, McCoy's 5A, RPMI 1640)
Fetal bovine serum (10%)
Penicillin-streptomycin-glutamine (1%)

controls

Positive control: Not specified
Negative control: Not specified

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