Participants were not required to fast before PET scans but rather to spend the day and hours prior to scanning according to their custom. Subjects were instructed to refrain from drinking alcohol for at least 24 h before PET scanning. If applicable, participants were asked to consume caffeine and nicotine within usual limits on the day of PET scanning.
PET images were acquired on a GE Advance scanner (General Electric Medical Systems, Milwaukee, WI) with a spatial resolution of six mm full-width at half-maximum (FWHM). Emission data were acquired over 90 min after bolus-injection of 302 ± 79 MBq (mean ± SD) (+)-[11C]-PHNO. Radiosynthesis was performed as described previously (Rami-Mark et al., 2013 (link), Pfaff et al., 2019 (link)). Employing filtered–back projection, images were reconstructed from sinograms to 15 one minute frames and 15 five minute frames. Attenuation correction was performed using matrices acquired immediately before tracer injection in a five minute transmission scan using a rotating 68Ge source. All scans were corrected for decay to the time of radioligand injection. T1 and proton density (PD) weighted 3 T magnetic resonance images (MRI) were acquired for PET image co-registration and delineation of regions of interest (ROI).
PET images were acquired on a GE Advance scanner (General Electric Medical Systems, Milwaukee, WI) with a spatial resolution of six mm full-width at half-maximum (FWHM). Emission data were acquired over 90 min after bolus-injection of 302 ± 79 MBq (mean ± SD) (+)-[11C]-PHNO. Radiosynthesis was performed as described previously (Rami-Mark et al., 2013 (link), Pfaff et al., 2019 (link)). Employing filtered–back projection, images were reconstructed from sinograms to 15 one minute frames and 15 five minute frames. Attenuation correction was performed using matrices acquired immediately before tracer injection in a five minute transmission scan using a rotating 68Ge source. All scans were corrected for decay to the time of radioligand injection. T1 and proton density (PD) weighted 3 T magnetic resonance images (MRI) were acquired for PET image co-registration and delineation of regions of interest (ROI).
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