Staphylococci Identification Protocol

All isolates were initially screened using Gram staining, catalase and coagulase tests. Those that demonstrated potential staphylococci characteristics were identified by Matrix-assisted laser desorption ionization time flight mass-spectroscopy (MALDI-TOF-MS, Microflex LT, Bruker Daltonics, Coventry, UK) in a positive linear mode (2000–20,000 m/z range) as described previously [12 (link)]. The resulting spectra were compared with reference spectra by using the Biotyper 3.0 software (Bruker Daltonics, Coventry, UK). Escherichia. coli DH5α (Bruker Daltonics, Coventry, UK) was used as a standard for calibration and quality control.

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Xu Z., Shah H.N., Misra R., Chen J., Zhang W., Liu Y., Cutler R.R, & Mkrtchyan H.V. (2018). The prevalence, antibiotic resistance and mecA characterization of coagulase negative staphylococci recovered from non-healthcare settings in London, UK. Antimicrobial Resistance and Infection Control, 7, 73.

Publication 2018

Microflex LT Biotyper 3.0 software Escherichia. coli DH5α

Catalase Coagulase Escherichia coli Maldi ms Staphylococci

Corresponding Organization : University of East London

Other organizations : Tianjin Medical University, Middlesex University, Natural History Museum, Huashan Hospital, Fudan University, Tianjin Hospital, Queen Mary University of London

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Variable analysis

independent variables

Gram staining
Catalase test
Coagulase test

dependent variables

Identification of potential staphylococci characteristics

control variables

Escherichia coli DH5α (used as a standard for calibration and quality control)

controls

Positive control: Escherichia coli DH5α (used as a standard for calibration and quality control)
Negative control: Not explicitly mentioned

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