Overexpression of CHD7 and PAX6 in Cells

Human full-length CHD7 cDNA (pF1KE9669 Flexi ORF Clone, PROMEGA, www. promega.jp) was subcloned into the pF5K CMV-neo Flexi vector vector (Promega), and a pMX-Pax6 plasmid (mouse) was obtained from Addgene (Cambridge, MA, www.addgene.org). Human PAX6 expression vector (pCS-PAX6, BC011953) was purchased from Transomic (Huntsville, AL, www.transomic.com). Recombinant lentiviruses were produced by the shCHD7 lentivirus vector (EHS4430-98514866, EHS4430-98714285, Open Biosystems. dharmacon.gelifesciences. com) or control shRNA vector (RHS4346, Open Biosystems). Retrovirus and lentivirus production including shMIF were performed as described previously [7 (link), 8 (link)].

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Ohta S., Yaguchi T., Okuno H., Chneiweiss H., Kawakami Y, & Okano H. (2016). CHD7 promotes proliferation of neural stem cells mediated by MIF. Molecular Brain, 9, 96.

Publication 2016

RHS4346

Cdna Clone Human Lentivirus Mouse Plasmid Promega Retrovirus Shrna Vector

Corresponding Organization : Keio University Hospital

Other organizations : Keio University, Centre National de la Recherche Scientifique, Neurosciences Paris-Seine, Sorbonne Université, Inserm, Institut de Biologie Paris-Seine

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Variable analysis

independent variables

ShCHD7 lentivirus vector
Control shRNA vector
Recombinant lentiviruses
Retrovirus and lentivirus production including shMIF

dependent variables

Not explicitly mentioned

control variables

Human full-length CHD7 cDNA (pF1KE9669 Flexi ORF Clone, PROMEGA, www. promega.jp)
PF5K CMV-neo Flexi vector vector (Promega)
PMX-Pax6 plasmid (mouse) obtained from Addgene (Cambridge, MA, www.addgene.org)
Human PAX6 expression vector (pCS-PAX6, BC011953) purchased from Transomic (Huntsville, AL, www.transomic.com)

positive controls

Not explicitly mentioned

negative controls

Control shRNA vector (RHS4346, Open Biosystems)

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