Western Blot Analysis of Splicing Factors

Western blot analyses were carried out as described (24 (link)) using antibodies against U2AF35 (10334–1-AP, Protein Tech Group), U2AF65 (U4758, Sigma), actin (ab37063, Abcam), tubulin (ab56676, Abcam) and CAPERα (PA5–31103, Thermo Fisher Scientific). AntiXpress antibodies were purchased from LifeTechnologies (R910–25). Antibodies against PUF60, hnRNP C and hnRNP I (PTB) were a generous gift of Adrian Krainer, Gideon Dreyfuss and Christopher Smith, respectively.

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Kralovicova J., Knut M., Cross N.C, & Vorechovsky I. (2015). Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3′ splice-site organization and activity of U2AF-related proteins. Nucleic Acids Research, 43(7), 3747-3763.

Publication 2015

U4758 ab56676 PA5–31103

Actin Antibodies Hnrnp Hnrnp c Protein Tubulin Western blot analyses

Corresponding Organization :

Other organizations : University of Southampton, Wessex Regional Genetics Laboratory, Salisbury District Hospital

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Variable analysis

independent variables

Antibodies against U2AF35
Antibodies against U2AF65
Antibodies against actin
Antibodies against tubulin
Antibodies against CAPERα
AntiXpress antibodies

dependent variables

Western blot analyses

control variables

Antibodies against PUF60
Antibodies against hnRNP C
Antibodies against hnRNP I (PTB)

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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